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Cloning and Expression
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Genomic DNA
Genomic DNA
Lambda DNA
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Lambda DNA
Lambda DNA from New England Biolabs
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Citations:
(90)
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New England Biolabs for
Lambda DNA
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Description
Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7). Lambda DNA is 48,502 base pairs in length.
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Lambda DNA
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
N3011L
Source/Expression System
Isolated from bacteriophage lambda (cI857ind 1 Sam 7)
Molecule Name
DNA
Quantity
1250 µg
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
DNA Type
Lambda DNA
Size Range
48.5 Kbp
Temperature
-20 C
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (90)
(1)
Genome sequence and characterization of a novel Pseudomonas putida phage, MiCath
Sci Rep
December 9, 2023
James Jaryenneh, Joseph S. Schoeniger, Catherine M. Mageeney
DNase I step followed by DNase inactivation and eluted in diH20. MiCath and Lambda (
NEB
, catalog no.
N3011S
) DNAs were digested with
EcoRI-HF
(
NEB
, catalog no.
R3101S
),
BamHI-HF
(
NEB
, catalog no.
R3136S
),
BstXI
(2)
Reentrant DNA shells tune polyphosphate condensate size
bioRxiv
September 20, 2023
Ravi Chawla, Jenna K. A. Tom, Tumara Boyd, Danielle A. Grotjahn, Donghyun Park, Ashok A. Deniz, Lisa R. Racki
2017)109",30152,BLR (F- ompT hsdSB(rB-mB-)gal dcm (DE3) Δ(srl-
recA
)306::Tn10 (TetR))
Lambda DNA
,
NEB
Catalog#
N3011L
,48502,- T4 DNA,"T4 GT7 DNA, Catalog# 318-03971, FUJIFILM Wako Chemicals USA",166000,- Table of plasmids
(3)
Metal Ions Modify In Vitro DNA Damage Yields with High-LET Radiation
Toxics
September 12, 2023
Dylan J. Buglewicz, Cathy Su, Austin B. Banks, Jazmine Stenger-Smith, Suad Elmegerhi, Hirokazu Hirakawa, Akira Fujimori, Takamitsu A. Kato
England BioLabs Inc., Ipswich, MA, USA, stock concentration of 500 ng/μL, 48,502 base pairs in length,
N3011S
) or 83 ng of single-strand DNA (ssDNA) of M13mp18
phage
(
New England BioLabs
Inc, stock concentration
(4)
Miniaturized Non-Contact Heating and Transmitted Light Imaging Using an Inexpensive and Modular 3D-Printed Platform for Molecular Diagnostics
Sensors (Basel)
September 7, 2023
Alex Laman, Debayan Das, Aashish Priye
Novagen, Cat. no. 71085-3), 50 μM of the SYTO82 dye, 1 µL of target DNA λ-
phage
template DNA (Cat. no.
N3011S
,
New England Biolabs
) and 67.8 µL of PCR grade water (HyClone, Logan, UT, USA; Cat. no. SH30538.02).
(5)
Microfluidic delivery of cutting enzymes for fragmentation of surface-adsorbed DNA molecules
PLOS ONE
September 6, 2023
Julia Budassi, NaHyun Cho, Anthony Del Valle, Jonathan Sokolov
produced in two steps. First, 200μl of a 50ng/μl solution (using
Lambda DNA
,
New England Biolabs
(
NEB
)
N3011S
), containing 1.5μl of the fluorescent dye SyBr Gold (Invitrogen,
S11494
,
Thermo Fisher Scientific
, Waltham,
(6)
DNA methylation protocol for analyzing cell-free DNA in the spent culture medium of human preimplantation embryos
STAR Protocols
June 1, 2023
Yuan Gao, Yidong Chen, Jie Qiao, Jin Huang, Lu Wen
oil,Vitrolife,10029 Hyaluronidase,
Sigma-Aldrich
,
H4272
Tris-EDTA,
Sigma-Aldrich
,
T9285
Triton X-100
,
Sigma-Aldrich
,T8787 1 M KCl,Sigma-Aldrich,60142
Lambda DNA
,
New England Biolabs
,
N3011S
Proteinase K
,Zymo,D3001-2-20 dNTP Mixture,TaKaRa,4019 "Klenow DNA polymerase (3′–5′ exo-, 50 U/μL)",TIANGEN,NG202-02 "Klenow
(7)
Robust membrane protein tweezers reveal the folding speed limit of helical membrane proteins
eLife
May 30, 2023
Seoyoon Kim, Daehyo Lee, W C Bhashini Wijesinghe, Duyoung Min
at one end and azide or 2×biotin at the other end, were amplified by
PCR
from λ DNA template (
NEB
,
N3011S
). The primers were added to total 8 ml
PCR
mixture – 1 µM of a forward primer (ACAGAAAGCCGCAGAGCA) with
(8)
A Microstrip Transmission Line Biosensor to Measure the Interaction between Microliter Aqueous Solutions and 1.0–17.0 GHz Radio Frequencies
Sensors (Basel)
May 30, 2023
Mary Godfrey, Daniel Ewert, Ryan Striker, Benjamin Braaten
(18.2 MΩ/cm @ 25 °C), Tris-EDTA Buffer,
New England Biolabs
lambda DNA
containing 1250 µg (Product #
N3011L
), iodized sodium chloride, Kimtech wipes, 70% Ethanol spray, and a digital 2-Channel K-Type Thermocouple
(9)
Etoposide promotes DNA loop trapping and barrier formation by topoisomerase II
Nature Chemical Biology
May 1, 2023
Tung T. Le, Meiling Wu, Joyce H. Lee, Neti Bhatt, James T. Inman, James M. Berger, Michelle D. Wang
(Extended Data Fig. 1a). The 12.7-kb DNA was amplified from
lambda DNA
(
New England Biolabs
(
NEB
),
N3011S
) using the primers CTACCCGAGTGCGTGACAT and CCAGTCTCGTGAAGCGGTA with Phusion DNA polymerase (
NEB
,
M0530S
).
(10)
Comprehensive benchmark and architectural analysis of deep learning models for nanopore sequencing basecalling
Genome Biology
April 11, 2023
Marc Pagès-Gallego, Jeroen de Ridder
Lambda phage
dataset that we sequenced. The
Lambda phage
genome DNA material was purchased from
NEB
(
N3011S
). The sample was prepared according to the ONT
Ligation
KIT and sequenced with a MinION flow cell. Data
(11)
Micromechanical Study of Hyperacetylated Nucleosomes Using Single Molecule Transverse Magnetic Tweezers
International Journal of Molecular Sciences
March 24, 2023
Santosh Gaire, Roberto L. Fabian, Raghabendra Adhikari, Pamela L. Tuma, Ian L. Pegg, Abhijit Sarkar
discussed in references [39,41]—see schematics in Figure 5. In brief, we start by functionalizing λ-DNA (
N3011S
,
New England Biolabs
, Ipswich, MA, USA), which involves ligating biotinylated DNA oligomers (five biotins)
(12)
A trailing ribosome speeds up RNA polymerase at the expense of transcript fidelity via force and allostery
Cell
March 16, 2023
Liang Meng Wee, Alexander B. Tong, Alfredo Jose Florez Florez Ariza, Cristhian Cañari-Chumpitaz, Patricia Grob, Eva Nogales, Carlos J. Bustamante
367-93-1 Kanamycin sulfate,Sigma-Aldrich,Cat#60615; CAS: 70560-51-9
Lambda Phage
DNA,
New England Biolabs
,Cat#
N3011S
; CAS: 91080-14-7 "Magnesium acetate tetrahydrate, (CH3COO)2Mg•4H2O",Sigma-Aldrich,Cat#M5661; CAS: 16674-78-5 "Magnesium
(13)
Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit
eLife
December 13, 2022
Shoji Tane, Keishi Shintomi, Kazuhisa Kinoshita, Yuko Tsubota, Makoto M Yoshida, Tomoko Nishiyama, Tatsuya Hirano
reagent,pUC19,Genbank: L9137,RRID: Addgene_50005, Recombinant DNA reagent,λDNA,
New England Biolabs
,
N3011S
, Sequence-based reagent,hCAP-H (CH) deletion-F,This paper,Forward primer,GAACGACTTCTCTACCGACTCTCCC Sequence-based
(14)
Topological digestion drives time-varying rheology of entangled DNA fluids
Nature Communications
December 1, 2022
D. Michieletto, P. Neill, S. Weir, D. Evans, N. Crist, V. A. Martinez, R. M. Robertson-Anderson
(kbp)), supplied from
New England Biolabs
at 0.5 mg/ml in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8) buffer (
N3011
), which we concentrate to 1 mg/ml via ethanol precipitation. We note that this construct has been used
(15)
Bloom helicase mediates formation of large single–stranded DNA loops during DNA end processing
Nature Communications
December 1, 2022
Chaoyou Xue, Sameer J. Salunkhe, Nozomi Tomimatsu, Ajinkya S. Kawale, Youngho Kwon, Sandeep Burma, Patrick Sung, Eric C. Greene
substrates were prepared as previously described17. For dsDNA with a 30-nt 3′-end tail, λ-DNA (
NEB
, Cat. No.
N3011S
) was annealed to a biotinylated oligo (5′-Phos-AGG TCG CCG CCC-3′BioTEG), and the second end of the
(16)
Control of λ Lysogenic Escherichia coli Cells by Synthetic λ Phage Carrying cIantisense
ACS Synthetic Biology
November 18, 2022
Ho Joung Lee, Hyun Ju Kim, Sang Jun Lee
h. Isolation, Propagation, and
Purification
of the
Phage
To isolate
bacteriophage
λ, 1 μg of λ DNA (
N3011
,
NEB
, Ipswich, MA, USA) was electroporated into
E. coli
MG1655.
Electroporation
was performed at 25
(17)
Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics
Nature Communications
October 23, 2022
MomÄilo Gavrilov, Joshua Y. C. Yang, Roger S. Zou, Wen Ma, Chun-Ying Lee, Sonisilpa Mohapatra, Jimin Kang, Ting-Wei Liao, Sua Myong, Taekjip Ha
\setlength{\oddsidemargin}{-69pt} \begin{document}$$\lambda$$\end{document}λ-DNA from
NEB
(catalog #
N3011S
) as a template and following primers generating 200 bp amplicon: 5′-CGGCTTCTGACTCTCTTTCC (forward) and 5′-
(18)
High-resolution phylogenetic and population genetic analysis of microbial communities with RoC-ITS
ISME Communications
October 10, 2022
Douglas B. Rusch, Jie Huang, Chris Hemmerich, Matthew W. Hahn
RoC-ITS 27 F Unique primer. The
PCR
was carried out with 1 μl of
Lambda DNA
(20 ng/μl; Catalog number:
N3011S
. Vendor:
New England Biolabs
Inc), 12.5 μl of KAPA 2X Master Mix, 1 μl of 10 μM Lambda_F_23S and the
(19)
Chromatin Liquid–Liquid Phase Separation (LLPS) Is Regulated by Ionic Conditions and Fiber Length
Cells
October 6, 2022
Qinming Chen, Lei Zhao, Aghil Soman, Anastasia Yu Arkhipova, Jindi Li, Hao Li, Yinglu Chen, Xiangyan Shi, Lars Nordenskiöld
via a Sephacryl S-1000 column. The
lambda DNA
(48,502 bp) were purchased from
New England Biolabs
(
N3011L
). 2.6.
Chromatin
Preparation Reconstitution of
nucleosome
arrays was conducted following the published
(20)
Facile detection of mechanical forces across proteins in cells with STReTCh
Cell Reports Methods
September 19, 2022
Brian L. Zhong, Vipul T. Vachharajani, Alexander R. Dunn
R3136
Biotin-PEG3-CoenzymeA,SiChem,Cat# SC-8618 SYPRO
Orange
,
ThermoFisher
,Cat#
S6650
Lambda phage
DNA,
New England Biolabs
,Cat#
N3011
HaloTag Succinimidyl Ester (O4) Ligand
,
Promega
,Cat#
P6751
Sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate,ThermoFisher,Cat#
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Lambda DNA from New England Biolabs
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